Coding

Part:BBa_K2230020:Design

Designed by: Yi-Lun Huang   Group: iGEM17_Mingdao   (2017-10-19)


ptsG/pSB1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 783
    Illegal NgoMIV site found at 1206
    Illegal AgeI site found at 490
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

The ptsG gene was amplified from gDNA of Salmonella typhimurium and cloned onto pSB1C3

References